Mechanism of Cinnamaldehyde Promoting the Apoptosis of RL95-2 Cells in Endometrial Carcinoma / 中山大学学报(医学科学版)

@#【Objective】To investigate the effect of cinnamaldehyde on the apoptosis of RL95- 2 cell in endometrial carcinoma. 【Methods】 Endometrial carcinoma RL95- 2 cells were treated with cinnamaldehyde，and the proliferation activity and IC50 of endometrial carcinoma RL95-2 cells were detected by MTT colorimetry assay. Apoptotic morphology was observed after Hoechst 33258 staining. The percentage of apoptosis in RL95-2 cells was measured by flow cytometry. Western blot analysis was used to test the effect of cinnamaldehyde on the expression of Cleaved caspase- 3，caspase-3， NF-κB·p65，IL-6 and IGF-R in RL95-2 cells.【Results】Cinnamaldehyde can reduce the viability rate of endometrial cancer RL95- 2 cells，which is related to the treatment duration and concentration. Compared with the solvent control group， the apoptosis percentage of RL95- 2 cells in the cinnamaldehyde group （0.29， 0.59， 1.20 mg/mL） was significantly increased after 48 hours（P < 0.01），typical apoptotic bodies were found ，and the expression of Cleaved caspase-3 protein was significantly increased（P < 0.01），there was no significant change in the expression of Caspase 3 protein（P > 0.05），while the expression of NF- κB · p65，IL- 6 and IGF- R protein were significantly increased（P <0.05）.【Conclusion】Cinnamaldehyde can reduce the expression of NF-κB·p65，IL-6 and IGF-R proteins in RL95-2 cells and promote the apoptosis of RL95-2 cells，thus playing an anti-endometrial cancer role.

Expressions of HO-2 and CO in the corpus cavernosum of castrated rats / 中华男科学杂志

<p><b>OBJECTIVE</b>To explore the expressions of HO-2 and CO in the corpus cavernosum of castrated rats in order to further study the pathogenesis of erectile dysfunction (ED).</p><p><b>METHODS</b>We randomly divided 72 male SD rats into four groups: normal control, sham operation, castration, and castration + ZnPP. We detected intracavernous pressure (ICP) and penile erection in the basic condition and after apomorphine (APO) induction, determined the expression of the HO-2 protein in the corpus cavernosum by laser scanning confocal microscopy, and measured the level of CO by spectrophotometry during different periods of penile erection.</p><p><b>RESULTS</b>The ICP in the basic condition and that after APO induction and the rate of penile erection were decreased significantly in the castration group ([11.68 ± 0.69] mmHg, [54.81 ± 3.86] mmHg, and 33.3%) and the castration + ZnPP group ([11.20 ± 0.71] mmHg, [41.17 ± 5.41] mmHg, and 22.2%) as compared with the normal control ([22.83 ± 2.66] mmHg, [66.92 ± 7.77] mm-Hg, and 100%) and the sham operation group ([23.35 ±2.22] mmHg, [70.43 ?7. 22] mmHg, and 100%) (all P <0. 01). After APO induction, ICP in the castration + ZnPP group was remarkably reduced in comparison with that in the castration group (P < 0.01), and so was the expression of the HO-2 protein before and during penile erection in the castration (445.4 ± 23.7 and 847.4 ± 35.0) and the castration + ZnPP group (390.1 ± 29.7 and 526.0 ± 52.5) compared with the normal control (512.7 ±57.4 and 1145.2 ± 89.8) and the sham operation group (583.7 ± 8.0 and 1016.3 ± 79.8), the expression of the HO-2 protein significantly decreased in the castration group (445.4 ± 23.7 and 847.4 ± 35.0) (P < 0.05 or 0.01), markedly lower in the castration + ZnPP than in the castration group during penile erection (P < 0.01) but with no significant differences among the four groups after it. Before, during and after penile erection, the levels of CO were remarkably decreased in the castration ([20.59 ± 1.01], [32.53 ± 1.26], and [18.71 ± 1.22] x 10(-7) nmol/L) and the castration +ZnPP group ([12.52 ± 1.05], [21.90 ± 1.02], and [16.56 ± 0.55] x 10(-7) nmol/L) as compared with the normal control ([26.76 ± 1.41], [48.25 ± 1.01], and [27.10 ± 1.58 ] x 10(-7) nmol/L) and the sham operation group ([25.41 ± 2.09], [ 47.90 ± 1.22], and [25.67 ± 1.20] x 10(-7) nmol/L) (P < 0.05 or 0.01), significantly lower in the castration + ZnPP than in the castration group during penile erection (P < 0.01).</p><p><b>CONCLUSION</b>Decreased expressions of HO-2 and CO may correlate with erectile dysfunction in castrated rats.</p>

A preliminary study of plasma microRNA levels in children with methylmalonic acidemia / 中国当代儿科杂志

<p><b>OBJECTIVE</b>To screen out differentially expressed microRNAs (miRNAs) in the plasma of children with methylmalonic acidemia (MMA), to determine the expression of miR-9-1 in plasma and to preliminarily evaluate the significance of miR-9-1 as a biomarker in MMA.</p><p><b>METHODS</b>Plasma was obtained from 17 MMA children, 10 hyperhomocysteinemia (HHcy) children without MMA (HHcy group), and 10 normal controls. Of 17 MMA children, 12 had HHcy (MMA+HHcy group), and 5 had no HHcy (MMA group). The differentially expressed miRNAs were screened out by miRNA microarray. Differentially expressed miR-9-1 was selected, and plasma miR-9-1 levels were determined by RT-PCR. Urine was collected from MMA patients who received vitamin B12 treatment, and plasma miR-9-1 levels were determined by RT-PCR after treatment.</p><p><b>RESULTS</b>The miRNA microarray analysis showed that 26 miRNAs were differentially expressed, among which 16 miRNAs (including miR-9-1) were down-regulated over 2 times, while 10 miRNAs were up-regulated over 2 times. The MMA+HHcy , MMA and HHcy groups had significantly down-regulated miR-9-1 compared with the normal control group (P<0.01). The patients who showed a good response to vitamin B12 treatment had significantly increased plasma miR-9-1 levels, without significant difference compared with the normal control group.</p><p><b>CONCLUSIONS</b>Plasma miR-9-1 is significantly down-regulated in MMA patients, but it is significantly up-regulated after vitamin B12 treatment, suggesting that miR-9-1 may act as a biomarker in monitoring the progression of MMA.</p>